Study on Physiological Variations in Certain Hemostatic Tests in University Students of Pakistan.
DOI:
https://doi.org/10.46568/bios.v7i1.235Keywords:
Clotting Time, Hemostasis, Clotting Time,Abstract
The present study was conducted to assess physiological variations in certain hemostatic tests, namely clotting time (CT) and prothrombin time (PT), among apparently healthy male and female university students in Pakistan, and to evaluate the relationship of these parameters with age, body weight, gender, and type of residence. Additionally, the study explored whether salinity% of platelet-free plasma could serve as an indirect indicator of fibrinogen concentration. A total of 184 students aged 20–30 years, enrolled in various four-year undergraduate programs, were included in the study. Body weight, temperature, pulse rate, and blood pressure were recorded prior to blood collection. Blood samples were collected aseptically and analyzed for clotting time using the Lee and White method (CT1) and the slide method (CT2), while prothrombin time (PT) was determined using Soluplastin reagent. Plasma salinity% was measured using a hand-held refractometer. Statistical analysis indicated that CT1, CT2, PT, and salinity% did not differ significantly (P ≥ 0.05) among different age and body weight groups. Regression analysis showed positive correlations of age with CT1 (r = 0.378) and CT2 (r = 0.292), while body weight was positively correlated with CT1 (r = 0.308) (P ≤ 0.05). Males and day scholars exhibited significantly higher CT1 values (P ≤ 0.05). Salinity% showed weak positive correlations with CT1 (r = 0.164), CT2 (r = 0.047), and PT (r = 0.147), although these associations were not statistically significant. These findings provide baseline information regarding physiological variations in selected hemostatic parameters among young Pakistani adults.
References
1. Monika G, Sameer S, Yogesh G, Preeya M, Krishnakant P. Comparison of BT (bleeding time)/CT (clotting time) with respect to blood group in medical students. Int J Health Sci Res. 2017;7(1):75-8.
2. Key NS, Geng JG, Bach RR. Tissue factor; from Morawitz to microparticles. Transactions of the American Clinical and Climatological Association. 2007;118:165.
3. Dorgalaleh A, Favaloro EJ, Bahraini M, Rad F. Standardization of prothrombin time/international normalized ratio (PT/INR). International journal of laboratory hematology. 2021 Feb;43(1):21-8.
4. Didisheim P. Tests of Blood Coagulation and Hemostasis: II. The Coagulation (Clotting) Time. JAMA. 1966 Dec 19;198(12):1299-1299.
5. Lee RI, White PD. A clinical study of the coagulation time of blood. The American Journal of the Medical Sciences (1827-1924). 1913 Apr 1;145(4):495.
6. Price P, Frey KB, Junge TL. Surgical technology for the surgical technologist: A positive care approach. Taylor & Francis; 2004.
7. Quick AJ. The prothrombin time in hemophilia and in obstructive jaundice. J Biol Chem. 1935;109:73–78.
8. Goeckel HJ. A Simple Method to Determine the Coagulation Time of the Blood. 1922: 122-123
9. Brummel KE, Butenas S, Mann KG. An integrated study of fibrinogen during blood coagulation. Journal of Biological Chemistry. 1999 Aug 6;274(32):22862-70.
10. Brass EP, Forman WB, Edwards RV, Lindan O. Fibrin formation: effect of calcium ions. Blood. 1978 Oct 1;52(4):654-8.
11. Jarrell DK, Vanderslice EJ, Lennon ML, Lyons AC, VeDepo MC, Jacot JG. Increasing salinity of fibrinogen solvent generates stable fibrin hydrogels for cell delivery or tissue engineering. PLoS One. 2021 May 19;16(5):e0239242.
12. Bamforth F. Extraction techniques and applications: biological/medical and environmental/forensics. In: Pawliszyn J, editor. Comprehensive Sampling and Sample Preparation. Vol. 1. Oxford: Elsevier; 2012. p. 1–16.
13. Parry BW. Laboratory evaluation of hemorrhagic coagulopathies in small animal practice. Veterinary Clinics of North America: Small Animal Practice. 1989 Jul 1;19(4):729-42.
14. Ratnoff OD, Colopy JE. A familial hemorrhagic trait associated with a deficiency of a clot-promoting fraction of plasma. The Journal of clinical investigation. 1955 Apr 1;34(4):602-13.
15. Sartim MA, Cezarette GN, Jacob-Ferreira AL, Frantz FG, Faccioli LH, Sampaio SV. Disseminated intravascular coagulation caused by moojenactivase, a procoagulant snake venom metalloprotease. International journal of biological macromolecules. 2017 Oct 1;103:1077-86.
16. Gabriel UU, Ezeri GN, Opabunmi OO. Influence of sex, source, health status and acclimation on the haematology of Clarias gariepinus (Burch, 1822). African Journal of Biotechnology. 2004;3(9).
17. Sivrikaya A, Baran H, Abusoglu S, Ozturk B, Vatansev H, Ali UN. Effect of gender and age on the prothrombin time (PT), activated partial thromboplastin time (aPTT) levels and international normalized ratio (INR). Int J Mevlana Med Sci. 2013;1(2):27-9.
18. de Brito Sousa JD, Sachett JA, de Oliveira SS, Mendonça-da-Silva I, Marques HO, de Lacerda MV, Fan HW, Monteiro WM. Accuracy of the lee–white clotting time performed in the hospital routine to detect coagulopathy in bothrops atrox envenomation. The American journal of tropical medicine and hygiene. 2018 Apr 2;98(5):1547.
19. Besi MA, Halada Y, Pasiga N. Description of the Results of the Clotting Time Examination of Lee and White Method in Diabetes Mellitus Patients at Toto Kabila Hospital. Jurnal Ilmiah dr. Aloei Saboe. 2021 Aug 2;1(2):87-93.
20. Hong X, Shan PR, Huang WJ, Zhu QL, Xiao FY, Li S, Zhou H. Influence of body mass index on the activated clotting time under weight‐based heparin dose. Journal of clinical laboratory analysis. 2016 Mar;30(2):108-13.
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Copyright (c) 2026 Umer Farooq, Dr. Zia ur Rehman, Mushtaq Hussain Lashari, Musadiq Idris, Saima Ilyas, Azka Kiran, Maryam Chaudry, Muhammad Noman Zahid, Javeria Ikram, Nasrullah Khan
This work is licensed under a Creative Commons Attribution 4.0 International License.
